Bacteria identification method and apparatus

ABSTRACT

The present invention discloses a bacteria-coding identification method,bacteria biochemical properties identifying papers which detect the biological properties using this method, and apparatus or means useful for identifying the genus and species of bacteria using the coding identification method and biochemical properties-identifying papers.

FIELD OF THE INVENTION

The invention involves a bacteria-coding identificaton method, which is for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilus, campylobacter, yeast-like fungi, corynebacteria, Micrococcaceae bacteria, Streptoccaceae bacteria, bacilli and lactobacilli, and bacteria biochemical properties-identifying papers which detect the biological properties using this method, i.e. identification papers for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilis, campylobacter, yeast-like fungi, corgnebacteria, Micrococcaceae bacteria, Streptoccaceae bacteria, bacilli and lactobacilli, and apparatus or means useful for identifying the genus and species of bacteria using the coding identification method and biochemical properties-identifying papers.

BACKGROUND OF THE INVENTION

Bacteria ideatification is a course including detecting the biological properties of the strain to be identificated using the bacteria biological properties assay device, and then according to the detection results of the biological properties of the strain, determining its genus and species by identification method and with the help of identification apparatus or identification coding retrieval means. At present, there are two kinds of bacteria identification met , which are double-branch identification and numerical identification, The former, which is well-known, achieves the identification of genus and species of the stain to be identified by analyzing the detection results of the biological properties one by one. The method contains too many identification steps and costs a lot time because the assay route and the next item of biological property to be detected cannot be determined until the detection result of the former one has been obtained and the steps are repeated again and again until the identification conclusion can be drawn. Meanwhile, the method Can not provide probability parameter when the bacteria identification result is shown because it is logical reasoning analytics. In order to decrease steps and reduce time, scholars abroad put forward numerical identification methods in succession, such as differentiation analysis method, nearest neighbor analysis method, Ochireed distance method, related coefficient method, probability most approximate value model method et. al.

These methods can simultaneously analyse several detection results of the biological properies of the strain to be identifed, which are obtained at one time, and provide probability parameter when the bacteria identification result is shown. Among the methods, probability most approximate value model identification method is applied at most and its effect is beat. Numerical identification methods, including probability most approximate value model method, are also called coding identification methods because in the course of bacteria identification by numerical methods several biological properties of bacteria usually need to be detected and the detection items or detection results need to be coded. There are 15 to 32 coding test items or sorts of test medium in the paper-hole respectively in the well-known coding identification methods or bacteria biochemical properties-identifying papers, such as VITEK, ATB, API, Micro-ID and Entertube II et al. Because of the unreasonable selection and inferior combination effect of the coding test items or the test medium sorts in the hole of bacteria biochemical properties-identifying papers, the identification of the bacteria identified for group is inefficient. Because the amount o coding test items or the medium sorts of biochemical properties-identifying papers is large and pats of coding test items have not identification value to identifying bacteria for group in the combination, the consumption and waste is age.

Different identification apparatus o identification coding retrieval means is used in the course of bacteria identification besides the use of different identification methods and bacteria biochemical properties-identifying papers. At present, bacteria identification device has special bacteria identifier with microprocessor as the core component and computer supported by bacteria identification software system. The well-known special bacteria identifier, such as VITEK Microbiological Automated Analyzer (VITEK Inc. U. S. A.) and ATB Bacteria Identifier (bio Merieux Inc., France), and computer supported by bacteria identification software system, such as API Advisory Computer, which identify bacteria by VITEK, ATB and API coding identification method respectively, exist the intrinsic defects of VITEK, ATB and API coding identification method mentioned above. Because the storage mediums of identification software are hard disc, floppy disc or tape, they have poor stability, physical vulnerability, complicated structure of themselves and the interface between them and microprocessor and large volume. Because there is no simulated color image on detection results of bacteria biological properties in the identification device, the man-machine interface is not visual and the operator can not obtain contrast promptness or analysis. Because there are less units of bacteria that can be identified in the identification device, the range of bacteria identification is narrow. Because the type of test system to detect the biological properties of the bacteria to be identified in ed in the identification system is single, the application is limited. Because there are many components in the identification device and the structure and interface are complicated, the volume is large, the cost of manufacture is high and the maintenance and upkeep are inconvenient. The well-known bacteria identification coding retrieval means, such as API Identification Coding Retrieval Manual and Micro-ID Identification Coding Retrieval Manual and the bacteria identification coding cetrieval computers which identity bacteria by API and Micro-ID coding identification method respectively, exist the intrinsic defects of API and Micro-ID coding identification method mentioned above.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a bacteria-coding identification method, which is for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilus, campylobacter, yeast-like fungi, corynebacteria, Micrococcaceae bacteria, Streptoccaceae bacteria, bacilli and lactobacilli, and bacteria biochemical properties-identifying papers which detect the biological properties using this method, i. e. identification papers for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilus, campylobacter, yeast-like fungi, corynebacteria, Micrococcaceae bacteria, Steptoccaceae bacteria, bacilli and lactobacilli, and apparatus or mean useful for identifying the genus and species of bacteria using the coding identification method and biochemical properties-identifying papers.

The above object of the present invention is realized in this way: First, according to the known bacteria biological properties, the coding test items for bacteria Identification are selected by optimum seeking method. Thereafter, bacteria biochemical properties-identifying papers are preparated on the basis of the optimum coding test items and are used to detect the biochemical properties of the strain to be identified. The detected bacteria biochemical properties are coed as biological code according to the method of 4-2-1 or 1-2-4 in octonal notation. Then the, identification conclusion on the genus and species of the strain to be identified is drawn from biological code by using numeral identification method and with the help of bacteria identification apparatus or by using bacteria identification coding retrieval means, which is obtained through procoding the probable test presentation or biological code of known bacteria in bacteria identification coding test items. To identify bacteria in different groups, the coding test items, the medium sorts of biochemical test in biochemical properties-identifying papers and the coding method of biological code are as the follows.

(1) To identify parenteral gram-negative bacilli, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 15 kinds of assimilation tests and test mediums, which are those of 2-keto-gluconate (2 KG), 3-hydroxy-benzoate (3HB), 3-hydroxy-butyrate (OBU), citrate (CIT), L-proline (LPR), histose (HIS), N-acetyl-D-glucosamine (NAG), glycogen (GLY), maltose (MA), sucrose (SUC), D-melibiose (MEL), L-fucose (FUC), D-glucose (GLU), inositol (IND) and manitol (MAN). The coding method is as follows.

    ______________________________________                                                2KG +   CIT +   NAG - SUC - GLG +                                       Coding 2HB -   LPR -   GLY + MEL - IND + Biological                            Method OBU +   HIS -   MAL + FUC - MAN + Code                                  ______________________________________                                         4-2-1  5       4       3     0     7     64807                                 1-2-4  6       1       6     0     7     61607                                 ______________________________________                                    

(2) To identify enteric gram-negative bacilli, the coding test items ate 15 tests, which are of those oxidase (OXI), growth in MCK agar (MCK), motility (MOT), lysine decarboxylase (LDC), ornithint decarboxylase (ODC), arginine dihydrolase (ADH), indole production (IND) urease (URE, citrate utilization (CIT), glucose oxidation (GLO), mannitol fermentation (MAN), sucrose fermentation (SUC), chamnose fermentation (RHA), amygdalin fermentation (AMY) arid sorbitol fermentation (SOR), the mediums in the paper-holes of biochemical properties-identifying papers are 12 sorts of test mediums, which are those of lysine decarboxylase (LDC), ornithint decarboxylase (ODC), arginine dihydrolase (ADH), indole production (IND), urease (URE). citrate utilization (CIT), glucose oxidation (GLO), mannitol fermentation (MAN), sucrose fermentation (SUC), chamnose fermentation (RHA), amygdalin fermentation (AMY) and sorbitol fermeatation (SOR). The coding method is as follows.

    ______________________________________                                                OXI -   LDC -   IND + OFO + RHA -                                       Coding MCK -   ODC +   URE + MAN + AMY - Biological                            Method MOT +   ADH -   CIT - SUC + SOR - Code                                  ______________________________________                                         4-2-1  1       2       6     7     0     12670                                 1-2-4  4       2       3     7     0     42370                                 ______________________________________                                    

(3) To identify Enterobacteriaceae bacteria, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 12 kinds of tests and test mediums, which are those of lysine decarboxylase (LDC), ornitine decarboxylase (ODC), malonate utilization (MAU), urease (URE), cellobiose fermentation (CEL), D-sorbitol fermentation (SOR), L-arabinose fermentation (LAR), adonitol fermentation (ADO), sucrose fermentation (SUC), dulcitol fermentation (DUL), α-methyl-D-glucoside fermentation (AMG) and D-mannitol fermentation (MAN). The coding method is as follows.

    ______________________________________                                                LDC +    URE +    LAR -  DUL -                                          Coding ODC -    CEL -    ADO +  AMG -  Biological                              Method MAU +    SOR -    SUC +  MAN -  Code                                    ______________________________________                                         4-2-1  5        4        3      0      6430                                    1-2-4  5        1        6      0      5160                                    ______________________________________                                    

(4) To identify anaerobic-bacteria, the coding test items are 14 tests which are those of gram stain (GRS), sporulation (SPO), α-glucoside (AGL), α-galactosidase (AGA), α-arabinosidase (AAR), β-glucosidase (BGL), β-galactosidase (BAG), β-glucuronidase (BGU), leucine arylamidase (LAA), proline arylamidase (PRA), alkaline phosphatase (ALP), indole production (IND), mannose fermentation (MAS) and β-N-acetyl-glucosaminidase (NAG), the mediums in the paper-holes of biochemical properties-identifying papers are 12 sorts of test mediums, which are those of α-glucosidase (AGL), α-galactosidase (AGA), α-arabinosidase (AAR), β-glucosidase (BGL), β-galactosidase (AGA), β-glucuronidase (BUG), leucine arylamidase (LAA), proline arylamidase (PRA), alkaline phosphatase (ALP), indole production (IND), mannose fermentation (MAS) and N-acetyl-β-D-glucosaminidase (NAG). The coding method is as follows:

    ______________________________________                                                GRS +   AGL -   BGL + LAA - IND -                                       Coding SPO -   AGA -   BGA - PRA + MAS + Biological                            Method --      ARA -   BGU + ALP + NAG - Code                                  ______________________________________                                         4-2-1  2       0       5     3     2     20532                                 1-2-4  1       0       5     6     2     10562                                 ______________________________________                                    

(5) To identify neisseria/hemophilus, the coding test items are 10 tests which are those of catalase (CAT), growth in Martin-Thayer medium (MTM), glucose fermentation (GLU), maltose fermentation (MLT), β-gluco-sidase (BGL), phenylphosphonate (OPS), glycine arylamidase (GLY), γ-glutamyl arylamidase (GGT), proline arylamidase (PRO) and reduction of resazurin (RES), the mediums in the paper-holes of biochemical properties-identifying papers are 9 sorts of test mediums, which are those of growth in Martin-Thayer medium (MTM), glucose fermeatation (GLU), maltose fermeatation (MLT), β-glucosidase (BGL), phenyl-phosphonate (OPS), glycine crylamidase (GLY), γ-glutamyl arylamidase (GGT), proline arylamidase (PRO) and reduction of resazurin (RES)-The coding method is as follows.

    ______________________________________                                                CAT +    MTM +    BGL -  GGT -                                          Coding --       GLU -    OPS +  PRO -  Biological                              Method --       MLT -    GLY +  RES +  Code                                    ______________________________________                                         4-2-1  1        4        3      1      1431                                    1-2-4  1        1        6      4      1164                                    ______________________________________                                    

(6) To itemdify campylobacter, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 9 kinds of test and test mediums, which are those of catalase (CAT), reduction of nitrate (NIT), urease (URE), succinate assimilation (SUT), acetate assimilation (ACE), hippurate hydrolysis (HIP) hydrogen sulfide production (HYS), γ-glutamyl transferase (GGT) and alkaline posphatase (ALP). The coding method is as follows.

    ______________________________________                                                  CAT+    SUC+       HYS-                                               Coding   NIT-    ACE-       GGT+  Biological                                   Method   URE-    HIP+       ALP-  Code                                         ______________________________________                                         4-2-1    4       5          2     452                                          1-2-4    1       5          2     152                                          ______________________________________                                    

(7) to identify yeast-like fungi, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 15 kinds of assimilation tests and test mediums, which are those of 2-keto -gluconate (2KG), actidione (ACT), erythritol (ERY), mannitol (MAN), inositol (INO), N-acetyl-glucosamine (NAG), L-arabinose (LAR), galactose (GAL), raffinose (RAF), cellobose (CEL) , lactose (LAC), maltose (MAL), melibiose (MEL), trehalose (TRE) and esculin (ESC). The coding method is as follows.

    ______________________________________                                                2KG +   MAN +   LAR - CEL - MEL -                                       Coding ACT -   INO -   GAL - LAC + TRE + Biological                            Method ERY -   NAG +   RAF + MAL + ESC - Code                                  ______________________________________                                         4-2-1  4       5       1     3     2     45132                                 1-2-4  1       5       4     6     2     15462                                 ______________________________________                                    

(8) To identify corynebacteria, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 9 kinds of test and test mediums, which are those of reduction of nitrate (NIT), urease (URE), esculin hydrolysis (ESC), L-maltose fermentation (MAL), sucrose fermentation (SUC), ribose fermentation (RIB) lactose fermentation (LAC), α-glucosidase (AGL) and β-galactosidase (BOA). The coding method is as follows.

    ______________________________________                                                  NIT+    MAL-       LAC-                                               Coding   URE-    SUC-       AGL+  Biological                                   Method   ESC+    RIB-       BGA+  Code                                         ______________________________________                                         4-2-1    5       4          3     543                                          1-2-4    5       1          6     516                                          ______________________________________                                    

(9) To identify Micrococcaceae bacteria, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 12 kinds of tests and test mediums, which are those of urease (URE), reduction of nitate (NIT), 3-hydroxy-butanone production (HYB), are dihydrolase (ADH), ornithine decarboxylase (ODC), alkaline phosphatase (ALP), β-galactosidase (BGA), β-glucuronidase (BGU), glucose fermentation (GLF), sucrose fermentation (SUC), mannitol fermentation (MAN) and trchalose fermentation. The coding method is as follows.

    ______________________________________                                                URE +    ADH -    BGA +  SUC -                                          Coding NIT +    ODC +    BGU -  MAN +  Biological                              Method HYB +    ALP +    GLF +  TRE -  Code                                    ______________________________________                                         4-2-1  7        3        5      2      7352                                    1-2-4  7        6        5      2      7652                                    ______________________________________                                    

(10) To identify streptoccaceae bacteria, the coding test items are 13 tests which are those of hemolysis (HEM) 3-hydroxy-butanone production (HYB), hippurate hydrolysis (HIP), arginine dihydrolase (ADH), α-leucine arylamidase (LAA), β-glucuronidase (BGU), β-galactosidase (BGA), ribose fermentation (RIB), raffinose fermentation (RAF), sorbitol fermentation (SOR) L-arabinose fermentation (LAR) and trehalose fermentation (TRE), the mediums in the paper-holes of biochemical propeties-identifying papers are 12 sorts of test mediums, which are those of 3-hydroxy-butanone production (HYB), hippurate hydrolysis (HIP), arginine dihydrolase (ADH), α-leucine arylamidase (LAA), β-glucuronidase (BGU), β-galactosidase (BGA), ribose fermentation (RIB), raffinose fermentation (RAF) sorbitol fermentation (SOR), L-arabinose fermentation (LAR) and trehalose fermentation (TRE). The coding method is as follows.

    ______________________________________                                                HEM +   HYB -   LAA - RIB + LAR -                                       Coding --      HIP -   BGU + RAF + TRE + Biological                            Method --      ADH +   BGA - SOR + LAC + Code                                  ______________________________________                                         4-2-1  1       1       2     7     3     11273                                 1-2-4  1       4       2     7     6     14276                                 ______________________________________                                    

(11) To identify bacilli, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 15 kinds of tests and test mediums, which are those of are dihydrolase (ADH), urease (URE), citrate utilization (CIT) β-glucosidase (BGL), starch hydrolysis (AMD), L-arabinose fermentation (LAR), ribose fermentation (RIB), sorbitol fermentation (SOR), D-tagatose fermeatation (DTA), N-acetyl-glucosamine fermeatation (NAG), fructose fermentation (FRU), ,mannose fermentation (MAS), galactose fermeatation (GAL), gentiobiose fermentation (GEN) and α-methyl-D-mannoside fermeatation (MDM). The coding method is as follows.

    ______________________________________                                                ADH +   BGA +   RIB - NAG - GAL -                                       Coding URE -   AMD -   SOR - FRU + GEN - Biological                            Method CIT -   LAR +   DTA - MAS - MDM + Code                                  ______________________________________                                         4-2-1  4       5       0     2     1     45021                                 1-2-4  1       5       0     2     4     15024                                 ______________________________________                                    

(12) To identify lactobacilli, the coding test items and the mediums in the paper-holes of biochemical properties-identifying papers are 15 kinds of tests and test mediums, which are those of arbutine fermentation (ARB), ribose fermentation (RIB), N-acetyl-glucosamine fermentation (NAG), D-xylose fermentation (DXY) trehalose formentation (TRE), sorbitol fermentation (SOR), sucrose fermentation (SUC), lactose fermentation (LAC), cellobiose fermentation (CEL), melibiose fermentation (MEL), L arabinose fermeatation (LAR), rhamnose fermentation (RHA), D-tagatose fermentation (DTA), gluconate fermentation (GNT) and starch hydrolysis (AMD). The coding method is as follows.

    ______________________________________                                                ARB -   DXY -   SUC - MEL + DTA -                                       Coding RIB +   TRE +   LAC - LAR + GTA - Biological                            Method NAG -   SOR +   CEL - RHA - AMD + Code                                  ______________________________________                                         4-2-1  2       3       0     6     1     23061                                 1-2-4  2       6       0     3     4     26034                                 ______________________________________                                    

The aforementioned bacteria identification apparatus as a whole consists of image sampling unit, input unit, pro unit, storage unit, output unit and software system. The image sampling unit contains COD (Charger Coupled Devices) pickup camera and image sampling circuit. With the support of software, the CCD pickup camera transforms color-changing image which embodies bacteria biological properties into video signal being transfered into image sampling circuit. The image sampling circuit transforms the video signal from CCD pickup camera into digital signal to be processed by processing unit. The input unit consists of keyboard, keyboard circuit and their interface. The keyboard consists of 46 keys, which are 10 numeral keys, i. e. 0 to 9, 26 alphabet keys, i. e. A to Z, and 10 symbol keys, i. e ▴, , , ▾, ←, →, +, -, CR, ES, The processing unit is 80286, 80386 or 80486 microprocessor and the relevant software. The storage unit uses the non-volatile semiconductor memory to store software and data. The non-volatile semiconductor memory is any one kind of programmable read-only memory, clearable programmable read-only memory or flash memory. The output unit ansists of color display and printer. The software consist of 4 system, which are system of identifeation, statistics, study and assistance. The identification system, which uses the present coding identification method as bacteria identification method, can identify 668 identification units of pathogen, conditional pathogen or opportunistic pathogen in 12 groups, which are common in clinic It can convert the inputted parameter of antibiotic sensitivity assay of the stain to be identified into sensitivity result, store the results of bacteria identification and antibiotic sensitivity assay and print the whole report list according to the request of the operator. In the course of bacteria identification, if there are several similar identification conclusions to a certain biological code or identification coding, the identification system can provide two kinds of identification pattern, which are direct identification, which draws the identification conclusion on genus and species of bacteria according to the identification value and standard likelihood value of the sin to be identified, and complementary identification, which identifies bacteria on the premise that the Identification system provides effective test items of complementary identification to the strain to be identified until the identification conclusion on genus and species of the strain is drawn. The study system in the systems software can study the test presentation of each identificaton unit in the identification system, the bacteria expression of the test presentation, the standard to explain the sensitivity extent of bacteria to antibiotics and the use method of the common used antibiotics. The assistance system in the system software can help the operator comprehensively understand every function of the identification apparatus, their use method and requests and can provide the operator color simulated image of test items of biological preporties of the strain to be identified and their combination selected by the operator to help him judge the test result correctly. The statistics system in the systems software can analyse the bacteria identification results, the assay results of antibiotic sensitivity and the data of patients et al stored by the user with 14 items of content to be statistically analysed including total detectable rate of various bacteria, detectable rate of various bacteria from different samples, detectable rate of various bacteria from different ages, detectable rate of various bacteria from different seasons, source distribution of the detectable bacteria, age distribution of the detectable bacteria, season distribution of the detectable bacteria, total drug-resistant rate of various bacteria, drug-resistant rate of the detectable bacteria from different samples, drog-resistant rate of the detectable bacteria from different ages, drug-resistant rate of the detectable bacteria from different seasons, source distribution of the drug-resistant strains age distribution of the drug-resistant strains, season distribution of the drug-resistant strains, and can output the analysis result by printing.

The aforementioned bacteria identification coding retrieval means is provided to determine the identification conclusion of genus and species of the strain to be identified and is obtained ed through precoding using numeral method according to the probable test presentation or biological coding of known bacteria in the combination of test items of the present oding identification method. It may be printed publication of Identification coding retrieval manual, computer coding retrieval system or computer analysis system. The bacteria identification coding retrieval mean is divided into 12 identification system according to the difference of biological groups of bacteria to be identified.

(1) identification coding retrieval system of parenteral gram-negative bacilli

(2) identification retrieval system of enteric gram-negative bacilli

(3) identification coding retrieval system of Enterobacteriaceae bacteria

(4) identification coding retrieval system of anaerobic bacteria

(5) identification coding retrieval system of neisseria/hemophilus

(6) identification coding retrieval system of campylobacteria

(7) identification coding retrieval system of yeast-like

(8) identification coding retrieval system of corynebacteria

(9) identification coding retrieval system of Micrococcaceae bacteria

(10) identification coding retrieval system of streptoccaceae bacteria

(11) identification coding retrieval system of bacilli

(12) identification coding retrieval system of lactobacilli

The coding test items and coding method of its biological code or identification code are as same as the aforementioned coding test items and coding method of identification system of bacteria in different groups. When the bacteria identification coding retrieval means is used to search the identification conclusion of genus and species of the strain to be identified, if there are several similar identification code, the bacteria identification coding retrieval system will provide effective complementary test items to further confirm the identification conclusion of tie strain to be identified

Using the present coding identification method and identification apparatus, the number of identification unit and the identification rate of 12 groups of bacteria are as follows:

(1) In the identification system of parenteral gram-negative bacilli, there are 104 identification units and the identifiable rate is 94.9775%.

(2) Ii the identification system of enteric gram-negative bacilli, there are 108 identification units and the identifiable rate is 95.6386%.

(3) In the identification system of Enterobaeriaceae bacteria, there are 97 identification units and the identifiable rate is 97.6804%

(4) In the identification system of anaerobic bacteria, there are 80 identification units and the identifiable rate is 97.2151%

(5) In the identification system of neisseria/hemophilus, there are 30 identification units and the identifiable rate is 95.632%

(6) In the identification system of campylobacter, there are 18 identification units and the identifiable rate is 77.1241%

(7) In the identification system of yeast-like fungi, there are 80 identification units and the identifiable rate is 95.3917%

(8) In the identification system of corynebacteria, there are 33 identification units and the identifiable rate is 96.4015%

(9) III the identification system of Micrococcaceae bacteria, there are 85 identification units and the identifiable rate is 96.9747%

(10) In the identification system of Streptoccaceae bacteria, there are 47 identification units and the identifiable rate is 95.7446%

(11) In the identification system of bacilli, there are 24 identification units and the identifiable rate is 81.8840%

(12) In the identification system of lactobacilli, there are 29 identification units and the identifiable rate is 86.6995%

In the present invention, because the aforementioned combination of test items and test mediums are used in the coding identification method and biochemical properties-identifying papers, every test in the combination has identificaton value and the identificaton of every group of bacteria is efficient; because the number of test items in the combination and the sorts of test mediums in biochemical properties-identify papers are little and each test item has identification value, and there is less consumption and no waste.

Because the structure of every unit in the present identification apparatus is as a whole, it has simple structure and interface, small volume, convenient installation and adjustment, good qualities of maintenance and upkeep. The image sampling unit contains CCD pickup camera and image sampling circuit. With the support of software, the CCD pickup camera transforms color-changing image which embodies bacteria biological properties into video signal being transfered into image sampling circuit. The image sampling circuit transforms the video signal from CCD pickup camera into digital signal to be prosed by pro units. So it operates directly, quickly, sensitively, accurately and has simple structure. Because the keyboard of the input unit consists of 46 keys, which are 10 numeral keys, i.e-0 to 9, 26 alphabet keys, i.e. A to Z, and 10 symbol keys, i.e ▴, , , ▾, ←, →, +, -, CR, ES, the operation of input is convenient. The storage medium of software system uses the non-volatile semiconductor memory, so it has simple structure and interface, small volume, high reliability and good stability and maintenance. The output unit consist of color display and printer, so the man-machine interface is effective and the needed data can be printed selectively according to the request of user.

Because the identification system in the software system of the present apparatus consists of 12 identification sub-systems, its identification range is extensive and it can identify 668 identification units of pathogen, conditional pathogen or opportunistic pathogen in 12 groups which are comon in dinic. Because the identification system consists of two kinds of identification pattern, which are direct identification and complementary identification, in the course of bacteria identification, if there are several similar identification conclusions to a certain biological code or identification coding, it can make selection according to the need and draw the exact identification conclusion. Because there is conversion system of assay parameter of antibiotic sensitivity of bacteria in the identification system, while the operator completes bacteria identification and assay of antibiotic sensitivity, he can input the assay parameter of antibiotic sensitivity into the system, which can automatically convert it into sensitivity extent and minimum inhibitory concentration range of the antibiotic to that bacterium when the system obtained the parameter. Because the identification system has functions of store and report, it can store the identification data and report the identification results according to the selection of the operator, and can provide the reader of the report the information of dinical pharmacology to help cure the bacteria in clinic when the results are reported. Because there is study system in the systems software, it can study the biological properties of every identification unit in the identification groups, the bacteria expression of test presentation, the standard to explain the assay results of antibiotic sensitivity and the use method of the common used antibiotics, and can conduct the contrast analysis of every two biological properties of every identification it. Because there is the assistance system in the systems software it can help the operator to understand, study and master every operation, function, selection and affect. Because there is statistics system in the software system, it can conduct every statistics and analysis to the stored identification data. Because color image of test results is allocated to study, assistance and identification system of the systems software, it can help the operator study or master the judgement standard and judgement method of various test results.

Because the aforementioned combination of test items is used in the present identification coding retrieval means, every test in the combination has identification value and the identification of every group of bacteria is efficient. Because the number of test items in the combination is little and every test item has identification value, there is less consumption and no waste because the, identificaton coding retrieval means consists of two kinds of identification pattern, which are direct identification and complementary identification, in the course of bacteria identification, if there ate several similar identification conclusions to a certain biological code, identifeation coding or a group of biological properties parameter, it can make selection according to the need and draw the correct identification conclusion.

The structure of the bacteria identification apparatus and the implementation examples, which are provided according to the present invention, are illustrated in detail as follows combining the additional figures of the directions.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is the sketch map of the structure of the bacteria identification apparatus.

FIG. 2 is the maim mean diagram of systems software of the bacteria identification apparatus.

DETAILED DESCRIPTION OF THE INVENTION I. Embodiment of the bacteria identification apparatus (FIG. 1)

The bacteria identification apparatus as a whole is assembled by joining CCD pickup camera with connectors and interface of image sampling circuit of image sampling unit (1) keyboard (46 keys including 10 numeral keys, i. e. 0 to 9, 26 alphabet keys, i. e A to Z, and 10 symbol keys, i. e. ▴, , , ▾, ←, →, +, -, CR, ES), keyboard circuit and its interface and connectors of input unit (3), microprocessor and its interface and connectors of prosing unit (4), memory and its interface and connectors of storage unit (2), color display and its circuit, interface and connectors and printer movement and its circuit, interface and connectors of output unit (5) each other, installing into the device box with power and power control switch, and writing software system into memory of storage unit (2) (See FIG. 1). The program block diagram of the software system is shown in FIG. 2.

II. Embodiment of Bacteria Identification Coding Retrieval Means.

(1) The bacteria identification coding retrieval manual is obtained by conducting precoding process to all biological codes of every system using numeral method according to the coding test items of bacteria identification and coding method of biological code, which are narrated in the directions, and making the results of precoding process into printed matter.

(2) The bacteria identification coding computer retrieval system is obtained by conducting precoding process to all biological codes of every system using numeral method according to the coding test items of bacteria identification and coding method of biological code, which are narrated in the directions, and storing the results of precoding process into floppy discs or hard disc of the computer.

(3) The bacteria identification coding compute analysis system is obtained by programming using numeral method according to the coding test items of bacteria identification and coding method of biological code, which are narrated in the directions.

III. Embodiment of Bacteria Biochemical Properties-Identifying Papers.

The a biochemical properties-identifying papers are made by preparing various test mediums in bacteria biochemical properties-identifying papers, adding them respectively into the holes of PVC material paper, which is shaped by injection using injection moulding machinary, and dried. The number of holes of PVC material paper in each kind of bacteria biochemical properties-identifying paper is as much as that of medium in the relevant bacteria biochemical properties-identifying paper, which is narrated in the directions. The sorts of medium are also as same as the narration in the directions.

IV. Embodiment of Bacteria Coding Identification Method

1. Embodiment of coding identification of parenteral gram-negative bacilli ##STR1## Coding identification test items:

    ______________________________________                                         1. 2KG     2-keto-Gluconate assimilation                                                  2-keto-gluconate assimilation test                                  2. 3HB     3-Hydroxy-Benzoate assimilation                                                3-hydroxy-benzoate assimilation test                                3. OBU     3-Hydroxy-Butyrate assimilation                                                3-hydroxy-butyrate assimilation test                                4. CIT     CITrate assimilation                                                           citrate assimilation test                                           5. LPR     L-PRoline assimilation                                                         l-proline assimilation test                                         6. HIS     HIStidine assimilation                                                         histidine assimilation test                                         7. NAG     N-Acetyl-d-Glucosamine assimilation                                            N-acetyl-d-glucosamine assimilation test                            8. GLY     GLYcogen assimilation                                                          glycogen assimilation test                                          9. MAL     MALtose assimilation                                                           maltose assimilation test                                           10. SUC    SUCrose assimilation                                                           SUCrose assimilation test                                           11. MEL    d-MELibiose assimilation                                                       D-melibiose assimilation test                                       12. FUC    l-FUCose assimilation                                                          L-fucose assimilation test                                          13. GLU    d-GLUcose assimilation                                                         D-glucose assimilation test                                         14. INO    INOsitol assimilation                                                          Dinositol assimilation test                                         15. MAN    MANnitol assimilation                                                          mannital assimilation test                                          ______________________________________                                    

Complementary test items:

    ______________________________________                                         16. RHA     RHAmnose assimilation                                                          rhamnose assimilation test                                         17. RIB     d-RIBose assimilation                                                          D-ribose assimilation test                                         18. ITA     ITAconate assimilation                                                         itaconate assimilation test                                        19. SUB     SUBerate assimilation                                                          suberate assimilation test                                         20. MNT     MaloNaTe assimilation                                                          malonate assimilation test                                         21. ACE     ACEtate assimilation                                                           acetate assimilation test                                          22. LAT     dl-LAcTate assimilation                                                        DL-lactate assimilation test                                       23. LAL     L-ALanine assimilation                                                         L-alanine assimilation test                                        24. SAL     SALicin assimilation                                                           salicin assimilation test                                          25. SOR     d-SORbitol assimilation                                                        D-sorbitol assimilation test                                       26. LAR     L-ARabinose assimilation                                                       L-arabinose assimilation test                                      27. PRO     PROpionate assimilation                                                        propionate assimilation test                                       28. CAP     CAPrate assimilation                                                           caprate assimilation test                                          29. VAL     VALeraTe assimilation                                                          valerate assimilation test                                         30. 5KG     5-keto-Gluconate assimilation                                                  5-keto-gluconate assimilation test                                 31. 4HB     4-Hydroxy-Benzoate assimilation                                                4-hydroxy-benzoate assimilation test                               32. LSE     L-SErine assimilation                                                          L-serine assimilation test                                         ______________________________________                                    

2. Embodiment of enteric gram-negative bacilli-coding identification method; ##STR2## Coding identification test items:

    ______________________________________                                         1* OXI        OXIdase                                                                        oxidase test                                                     2* MCK        growth in MCK agar                                                             growth in MCK agar test                                          3* MOT        MOTility                                                                       motility test                                                    4* LDC        Lysine DeCarboxylase                                                           lysine decarboxylase test                                        5. ODC        Ornithint Decarboxylase                                                        ornithint decarboxylase test                                     6. ADH        Arginine DiHydrolase                                                           arginine dihydrolase test                                        7. IND        INDole production                                                              indole production test                                           8. URE        UREase                                                                         urease test                                                      9. CIT        CITrate utilization                                                            citrate utilization test                                         10. GLO       GLucose Oxidation                                                              glucose oxidation test                                           11. MAN       MANnitol fermentation                                                          mannitol fermentation test                                       12. SUC       SUCrose fermentation                                                           sucrose fermentation test                                        13. RHA       RHAmnose fermentation                                                          rhamnose fermentation test                                       14. AMY       AMYgdalin fermentation                                                         amygdalin fermentation test                                      15. SOR       SORbitol fermentation                                                          sorbitol fermentation test                                       ______________________________________                                    

Complementary test items:

    ______________________________________                                         16. BGA     Beta-GAlactosidase                                                             β-galactosidase test                                          17. NIT     reduction of NITrate                                                           reduction of nitrate test                                          18. HIS     Hydrogen Sulfide production                                                    hydrogen sulfide production test                                   19. TDA     Tryptophane DesAminase                                                         tryptophane desaminase test                                        20. HYB     3-Hydroxy-2-Butanone production                                                3-hydroxy-2-butanone production test                               21. GEL     GELatinase                                                                     gelatinase test                                                    22. GLU     GLUcose Fermentation                                                           glucose fermentation test                                          23. INO     INOsitol fermentation                                                          inositol fermentation test                                         24. MEL     MELibiose fermentation                                                         melibiose fermentation test                                        25. ARA     ARAbinose fermentation                                                         arabinose fermentation test                                        26. NIG     NItrogen Gas production                                                        nitrogen production test                                           27 GLF      Glucose Fermentation                                                           glucose fermentation test                                          ______________________________________                                    

3. Embodiment of Enterobacteriaceae bacteria-coding identification method

Coding test items of Interobacteriaceae bacteria ##STR3## Coding identification test items:

    ______________________________________                                         1. LDC     Lysine DeCarboxylase                                                           lysine decarboxylase test                                           2. ODC     Ornitine Decarboxylase                                                         ornitine decarboxylase test                                         3. MAU     MAlonate Utilization                                                           malonate utilization test                                           4. URE     UREase                                                                         urase test                                                          5. CEL     CELlobiose fermentation                                                        cellobiose fermentation test                                        6. SOR     D-SORbitol fermentation                                                        D-sorbitol fermentation test                                        7. LAR     L-ARabinose fermentation                                                       L-arabinose fermentation test                                       8. ADO     ADOnitol fermentation                                                          adonitol fermentation test                                          9. SUC     SUCrose fermentation                                                           sucrose fermentation test                                           10. DUL    DUlcitol fermentation                                                          dulcitol fermentation test                                          11. AMG    Alpha-Methyl-D-Glucoside fermentation                                          α-methyl-D-glucoside fermentation test                        12. MAN    D-MANnitol fermentation                                                        D-mannitol fermentation test                                        ______________________________________                                    

Complementary test items:

    ______________________________________                                         13. IND  INDole production                                                              indole production test                                                14. MER  MERhyl red                                                                     merhyl red production test                                            15. HYB  3-Hydroxy-2-Butanone production                                                3-hydroxy-2-butanone production test                                  16. CIT  CITrate (Simons)                                                               citrate utilization test                                              17. HYS  Hydrogen Sulfide (TSI)                                                         hydrogen sulfide production test                                      18. PHE  PHEnylalanine deaminase                                                        phenylalanine deaminase test                                          19. ADH  Arginine DiHydrolase                                                           arginine dihydrolase test                                             20. MOT  MOTility                                                                       motility test                                                         21. GEL  GELatin hydrolysis                                                             gelatin hydrolysis test                                               22. KCN  growth in KCN                                                                  growth in KCN test                                                    23. GLU  D-GLUcose, acid                                                                D-glucose fermentation test with acid production                      24. GLG  D-GLucose, Gas                                                                 D-glucose fermentation test with gas production                       25. LAC  LACtose fermentation                                                           lactose fermentation test                                             26. SAL  SALicin fermentation                                                           salicin fermentation test                                             27. INO  myo-INOsitol fermentation                                                      myo-inositol fermentation test                                        28. RAF  L-RAFfinose fermentation                                                       L-raffinose fermentation test                                         29. RHA  L-RHAmnose fermentation                                                        L-rhamnose fermentation test                                          30. MAL  MALtose fermentation                                                           maltose fermentation test                                             31. DXY  D-xylose fermentation                                                          D-xylose fermentation test                                            32. TRE  TREhalose fermentation                                                         trehalose fermentation test                                           33. ERY  ERYthitol fermentation                                                         erythitol fermentation test                                           34. ESC  ESCulin hydrolysis                                                             esculin hydrolysis test                                               35. MEL  MELibiose fermentation                                                         melibiose fermentation test                                           36. ART  D-ARabitol fermentation                                                        D-arabitol fermentation test                                          37. GLY  GLYcerol fermentation                                                          glycerol fermentation test                                            38. MUC  MUCate fermentation                                                            mucate fermentation test                                              39. TRA  TRAtrate, Jordon's                                                             tartrate utilization test                                             40. ACE  ACEtate utilization                                                            acetate utilization test                                              41. LIP  LIPase (corn oil)                                                              Lipase (corn oil) test                                                42. DNA  DNAse                                                                          DNase test                                                            43. NIT  reduction of NITrate                                                           reduction of nitrate test                                             44. OXI  OXIdase, kovuc's                                                               exidase test                                                          45. BGA  Beta-GAlacotosidase                                                            β-galacotosidase test                                            46. YEL  YELlow pigment                                                                 yellow pigment production test                                        47. MAS  D-MAnnoSe fermentation                                                         D-Mannose fermentation test                                           ______________________________________                                    

4. Embodiment of anaerobic bacteria-coding identification method:

Coding test items of anaerobic bacteria ##STR4## Coding identification test items;

    ______________________________________                                         1 GRS      GRam Stain                                                                     gram stain test                                                     2 SPO      SPOrulation                                                                    sporulation test                                                    3. AGL     Alpha-GLUcosidase                                                              α-glucosidase test                                            4. AGA     Alpha-GAlactosidase                                                            α-galactosidase test                                          5 AAR      Alpha-ARabinosidase                                                            α-arabinasidase test                                          6. BGL     Beta-Glucosidase                                                               β-glucosidase test                                             7. BGA     Beta-GAlactosidase                                                             β-galactosidase test                                           8. BGU     Beta-GlucUronidase                                                             β-glucuronidase test                                           9. LAA     Leucine ArylAmidase                                                            leucine aryamidase test                                             10. PRA    PRoline Arylamidase                                                            proline arylamidase test                                            11. ALP    ALKaline Phosphatase                                                           alkaline phosphatase test                                           12. IND    INDole production                                                              indole production test                                              13. MAS    MAnnose fermentation                                                           mannose fermentation test                                           14. NAG    N-Acetyl-β-d-Glucosaminidase                                              N-acetyl-β-D-Glucosaminidase test                              15. URE    UREase                                                                         urase test                                                          16. ADH    Arginine DiHydrolase                                                           arginine dihydrolase test                                           17. BGP    Beta-Galactosidase-6-phosphate                                                 β-galactosidose-6-phosphate test                               18. RAF    RAFfinose fermentation                                                         raffinose fermentation test                                         19. NIT    reduction of NITrate                                                           reduction of nitrate test                                           20. AAA    Argininc ArylAmidase                                                           arginine arylamidase test                                           21. LGA    Leucyl Glycine Arylamidase                                                     leucyl glycine arylamidase test                                     22. PAA    Phenylalanine Arylamidase                                                      phyenylalanine aryamidase test                                      23. PYA    Pyroglutamic ac. Arylamidase                                                   pyroglutamic acid arylamidase test                                  24. TAA    Tyrosine Arylamidase                                                           tyrosine anglamidase test                                           25. ALA    Alanine Arylamidase                                                            alanine arylamidase test                                            26. GAA    Glycine ArylAmidase                                                            glycine arylamidase test                                            27. GAD    Glutamic Ac. Decarboxylase                                                     glutamic acid decarboxy lase test                                   28. AFU    Alpha-FUcosidase                                                               α-fucosidase test                                             29. HAA    Histidine ArylAmidase                                                          histidine arylamidase test                                          30. GGA    GLutamyl GLutamic ac. Arylmidase                                               glutamyl glutamic acid arylmidase test                              31. SAA    Serine ArylAmidase                                                             serine arylamidase test                                             32. MOR    MORphology                                                                     morphology test                                                     ______________________________________                                    

5. Embodiment of neisseria/hemophilus-coding identification method:

Coding test items of neisserial hemophilus ##STR5## Coding test items

    ______________________________________                                         1* CAT      CATalase                                                                       catalase test                                                      2. MTM      Martin-Thayer Medium                                                           growth in Martin-Thayer medium test                                3. GLU      GLUcose                                                                        glucose fermentation test                                          4. MLT      MaLTose fermentation                                                           maltose fermentation test                                          5. BGL      Beta-GLucosidase                                                               β-glucosidase test                                            6. OPS      PhenylphoSphonate                                                              phenylphosphonate test                                             7. GLY      GLYcine-p-nitroanilide                                                         glycine-p-nitroanilide test                                        8. GGT      Gamma-GLutarmyl-p-niTroanilide                                                 γ-glutarmyl-p-nitroamilide test                              9. PRO      PROline-p-nitroaniclide                                                        proline-p-nitroanilide test                                        10. RES     RESazurin                                                                      resazurin test                                                     ______________________________________                                    

Complimentary test items

    ______________________________________                                         11. ALA     ALanine-penitroanilide                                                         alanine-penitroanilide test                                        12. LYS     LYSine-p-nitroanilide                                                          lysine-p-nitroanilide test                                         13. PNC     P-Nitrophenyl-phosphonyl-cholinr                                               p-nitrophenyl-phosphonyl-cholinr test                              14. SUC     SUCrose fermentation                                                           sucrose fermentation test                                          15. TTZ     TriphenylTetraZolium                                                           triphenyltetrazolium test                                          16. ORN     ORNithine decarboxylase                                                        ornithine decarboxylase test                                       17. URE     UREase                                                                         urase test                                                         18. PEN     PENicillin G Sensibility                                                       penicillin g sensibility test                                      ______________________________________                                    

6. Embodiment of campylobacter-coding identification method

Test items of campybacter ##STR6## Coding test items:

    ______________________________________                                         1. CAT       CATalase                                                                       catalase test                                                     2. NIT       reduction of NITrate                                                           reduction of nitrate test                                         3. URE       UREase                                                                         urease test                                                       4. SUT       SUccinaTe assimilation                                                         succinate assimilation test                                       5. ACE       ACEtate assimilation                                                           acetate assimilation test                                         6. HIP       HIPpurate hydrolysis                                                           hippurate hydrolysis test                                         7. HYS       HYdrogen Sulfide production                                                    hydrogen sulfide production test                                  8. GGT       Gama-GLutamyl Transferase                                                      γ-glutamyl transferase test                                 9. ALP       ALPaline phosphatase                                                           alkaline phosphatase test                                         ______________________________________                                    

Complementary test items:

    ______________________________________                                         10. EST   ESTerase                                                                       esterase test                                                        11. TTC   reduction chlorure Triphenyl Transferase                                       reduction chlorure triphenyl transferase test                        12. PYR   PYRrolidonyl arylamidase                                                       pyrrolidonyl arylamidase test                                        13. ARY   L-ARYinine arylamidase                                                         L-aryinine arylamidase test                                          14. ASP   L-ASPartate arylamidase                                                        L-aspartate arylamidase test                                         15. GLU   GLUcose assimilation                                                           glucose assimilation test                                            16. NAL   NALidixigue sensibility                                                        nalidixique sensibility test                                         17. CFZ   CeFazoline sensibility                                                         cefazoline sensibility test                                          18. PRO   PROpinate assimilation                                                         propinate assimilation test                                          19. MLT   MaLaTe assimilation                                                            malate assimilation test                                             20. CIT   CITrate assimilation                                                           citrate assimilation test                                            21. ERS   ERythomycine Sensibility                                                       erythomycine sensibility test                                        ______________________________________                                    

7. Embodiment of yeast-like fungi-coding identification method:

Coding test items of yeast-like fungi ##STR7## Coding test items:

    ______________________________________                                         1. 2KG     2-keto-Gluconate assimilation                                                  2-keto-gluconate assimilation test                                  2. ACT     ACTidone assimilation                                                          actidone assimilation test                                          3. ERY     ERYthritol assimilation                                                        erythritol assimilation test                                        4. MAN     MANnitol assimilation                                                          mannitol assimilation test                                          5. INO     INOsitol assimilation                                                          inositol assimilation test                                          6. NAG     N-Acetnyl-d-Glucosamine assimilation                                           N-acetnyl-D-glucosamine assimilation test                           7. LAR     L-ARabinose assimilation                                                       L-arabinese assimilation test                                       8. GAL     GALactose assimilation                                                         galactose assimilation test                                         9. RAF     RAFfinose assimilation                                                         roffinose assimilation test                                         10. CEL    CELlobose assimilation                                                         cellobose assimilation test                                         11. LAC    LACtose assimilation                                                           lactose assimilation test                                           12. MAL    MALtose assimilation                                                           maltose assimilation test                                           13. MEL    MELibiose assimilation                                                         melibiose assimilation test                                         14. TRE    TREhalose assimilation                                                         trehalose assimilation test                                         15. ESC    ESCulin assimilation                                                           esculin assimilation test                                           ______________________________________                                    

Complementary test items

    ______________________________________                                         16. SUC    SUCrose assimilation                                                           sucrose assimilation test                                           17. LST    DL-LAcTate assimilation                                                        DL-lactate assimilation test                                        18. MDG    α-Methyl-D-Glucoside assimilation                                        α-methyl-D-glucoside assimilation test                        19. SOR    SORbitol assimilation                                                          sorbitol assimilation test                                          20. DXY    D-xylose assimilation                                                          D-xylose assimilation test                                          21. RIB    RIBose assimilation                                                            ribose assimilation test                                            22. GLY    GLYcerl assimilation                                                           glycerl assimilation test                                           23. RHA    RHAmnose assimilation                                                          rhamnose assimilation test                                          24. PLF    PaLatinosE assimilation                                                        palatinose assimilation test                                        25. MLZ    MeLeZitose assimilation                                                        melezitose assimilation test                                        26. GRT    GLucuRonaTe assimilation                                                       glucuronate assimilation test                                       27. GNT    GlucoNaTe assimilation                                                         gluconate assimilation test                                         28. LVT    LeVullinaTe assimilation                                                       levullinate assimilation test                                       29. GLU    GLUcose assimilation                                                           glucose assimilation test                                           30. SBE    SorBosE assimilation                                                           sorbose assimilation test                                           31. GLN    GLucosamiNe assimilation                                                       glucosamine assimilation test                                       ______________________________________                                    

8. Embodiment of congnebacteria-coding identification method

Coding test items of corynebacteria ##STR8## Coding test items

    ______________________________________                                         1. NIT      reduction of NITrate                                                           reduction of intrate test                                          2. URE      UREase                                                                         urease test                                                        3. ESC      ESCulin                                                                        esculin test                                                       4. MAL      l-MALtose fermentation                                                         L-maltose fermentation test                                        5. SUC      SUCrose fermentation                                                           sucrose fermentation test                                          6. RIB      RIBose fermentation                                                            ribose fermentation test                                           7. LAC      LACtose fermentation                                                           lactose fermentation test                                          8. AGL      Alpha-GLucosidase                                                              α-glucosidase test                                           9. BGA      Beta-GAlactosidase                                                             β-galactosidase test                                          10. PYZ     PYraZinamidase                                                                 pyrazinamidase test                                                11. PYR     PYRrolidonyl arylamidse                                                        pyrrolidonyl arylamidse test                                       12. ALP     ALkaline phosphate                                                             alkaline phosphate test                                            13. BGU     Beta-GlucUronidase                                                             β-glucuronidase test                                          14. NAG     N-Acetyl-β-Glucosamindase                                                 N-acetyl-β-D-glucosamindase test                              15. GEL     GELatine hydrolysis                                                            gelatine hydrolysis test                                           16. GLF     GLucose Fermentation                                                           glucose fermentation test                                          17. XYL     XYLose fermentation                                                            xylose fermentation test                                           18. MAN     MANitol fermentation                                                           manitol fermentation test                                          19. GLY     GLYcogen fermentation                                                          glycogen fermentation test                                         20. CAT     CATalase                                                                       catalase Test                                                      ______________________________________                                    

9. Embodiment of Micrococcaceae bacteria-coding identification method

Coding test items of micrococcaceae bacteria ##STR9## Coding test items:

    ______________________________________                                         1. URE        UREase                                                                         urease test                                                      2. NIT        reduction of NITrate                                                           reduction of nitrate test                                        3. V--P       acetion production                                                             acetion production test                                          4. ADH        Arginine DiHydrolase                                                           arginine dihydrolase test                                        5. ODC        Ornithine DeCarboxylase                                                        ornithine decarboxylase test                                     6. ALP        ALkaline phosphatase                                                           alkaline phosphatase test                                        7. BGA        Beta-GAlactosidase                                                             β-galactosidase test                                        8. BGU        Beta-GlucUronidase                                                             β-glucuronidase test                                        9. GLF        GLucose Fermentation                                                           glucose fermentation test                                        10. SUC       SUCrose fermentation                                                           sucrose fermentation test                                        11. MAN       MANnitol fermentation                                                          mannitol fermentation test                                       12. TRE       TREhalose fermentation                                                         trehalose fermentation test                                      ______________________________________                                    

Complementary test items:

    ______________________________________                                         13. ESC    ESCulin hydrolysis                                                             esculine hydrolysis test                                            14. FRU    FRUctose fermentation                                                          fructose fermentation test                                          15. MAS    MAnnoSe fermentation                                                           mannose fermentation test                                           16. MAL    MALtose fermentation                                                           maltose fermentation test                                           17. LAC    LACtose fermentation                                                           lactose fermentation test                                           18. RAF    RAFfinose fermentation                                                         raffinose fermentation test                                         19. ARG    ARGinine argiamidase                                                           arginine argiamidase test                                           20. PYR    PYRrolidonyl arylamidase                                                       pyrrolidonyl arylamidase test                                       21. NOV    NOVobocin (Resistance)                                                         novobiocin test                                                     22. NAG    N-Acetyl-GLUcosamine Fermentation                                              N-acetyl-glucosamine fermentation test                              23. TUR    TURranose fermentation                                                         turanose fermentation test                                          24. ARA    ARAbinose fermentation                                                         arobinose fermentation test                                         25. RIB    RIBose fermentation                                                            ribose fermentation test                                            26. CEL    CELlubiose fermentation                                                        cellubiose fermentation test                                        ______________________________________                                    

10. Embodiment of Streptoccaceae bacteria-coding identification method:

Coding test items of Streptoccaceae bacteria ##STR10## Coding test items:

    ______________________________________                                         1* HEM       HEMolysis                                                                      hemolysis test                                                    2. V--P      aceton production                                                              aceton production test                                            3. HIP       HIPpurate hydrolysis                                                           hippurate hydrolysis test                                         4. ADH       Arginine DiHydrolase                                                           arginine dihydrolase test                                         5. LAA       α-Leucine ArylAmidase                                                    α-leucine arylamidase test                                  6. BGU       Beta-GLucUronidase                                                             β-glucuronidase test                                         7. BGA       Beta-GAlactosidase                                                             β-galactosidase test                                         8. RIB       RIBose fermentation                                                            ribose fermentation test                                          9. RAF       RAFfinose fermentation                                                         raffinose fermentation test                                       10. SOR      SORbitol fermentation                                                          sorbitol fermentation test                                        11. LAR      L-ARabinose fermentation                                                       L-arabinose fermentation test                                     12. TRE      TREhalose fermentation                                                         trehalose fermentation test                                       13. LAC      LACtose fermentation                                                           lactose fermentation test                                         ______________________________________                                    

Complementary test items:

    ______________________________________                                         14. ESC      ESCulin hydrolysis                                                             esculin hydrolysis test                                           15. PYR      Pyrrolidonyl arylamidase                                                       pyrrolidonyl arylamidase test                                     16. AGA      Alpha-GAlactosidase                                                            α-GAlactosidase test                                        17. ALP      ALkalische phosphatase                                                         alkalische phosphatase test                                       18. MAN      MANnitol fermentation                                                          mannitol fermentation test                                        19. INU      INUlin fermentation                                                            inulin fermentation test                                          20. AMD      Starch                                                                         starch hydrolysis test                                            21. GLY      GLYcogen fermentation                                                          glycogen fermentation test                                        ______________________________________                                    

11. Embodiment of bacilli-coding identification method:

Coding test items of bacilli ##STR11## Coding test items:

    ______________________________________                                         1. ADH      Arginine DIHydrolase                                                           arginine dihydrolase test                                          2. URE      Urase                                                                          urase test                                                         3. CIT      CITrate utilization                                                            citrate utilization test                                           4. ONP      Ortho-Nitro-phenyl-Galactoside                                                 ortho-nitro-phenyl-galactoside test                                5. AMD      Starch                                                                         starch hydrolysis test                                             6. LAR      L-ARabircose fermentation                                                      L-arabircose fermentation test                                     7. RIB      RIBose fermentation                                                            ribose fermentation test                                           8. SOR      SORbitol fermentation                                                          sorbitol fermentation test                                         9. DTA      D-TAgatose fermentation                                                        D-tagatose fermentation test                                       10. NAG     N-Acetyl-Glucoseamine fermentation                                             N-acetyl-glucoseamine fermentation test                            11. FRU     FRUctose fermentation                                                          fructose fermentation test                                         12. MAS     MAnnoSe fermentation                                                           mannose fermentation test                                          13. GAL     GALactose fermentation                                                         galactose fermentation test                                        14. GEN     GENtiobiose fermentation                                                       gentiobiose fermentation test                                      15. MDM     α-Methyl-D-Mannoside fermentation                                        α-methyl-D-mannoside fermentation test                       ______________________________________                                    

Complementary test items:

    ______________________________________                                         16. GLY    GLYcerol fermentation                                                          glycerol fermentation test                                          17. ERY    ERYthritol fermentation                                                        erythritol fermentation test                                        18. DAR    D-ARabinose fermentation                                                       D-arabinose fermentation test                                       19. DXY    D-XYlose fermentation                                                          D-xylose fermentation test                                          20. LXY    L-XYlose fermentation                                                          L-xylose fermentation test                                          21. ADO    ADOnitol fermentation                                                          adonitol fermentation test                                          22. MDX    β-Methyl-D-Xyloside fermentation                                          β-methyl-D-xyloside fermentation test                          23. GLF    GLucose Fermentation                                                           glucose fermentation test                                           24. SOS    SOrboSe fermentation                                                           sorbose fermentation test                                           25. RHA    RHAmnose fermentation                                                          rhamnose fermentation test                                          26. DUL    DUlcitol fermentation                                                          dulcitol fermentation test                                          27. INO    INOsitol fermentation                                                          inositol fermentation test                                          28. MAN    MANnitol fermentation                                                          mannitol fermentation test                                          29. MDM    Methyl-D-Mannoside fermentation                                                α-methyl-D-mannoside fermentation test                        30. MDG    α-Methyl-D-Glucoside fermentation                                        α-methyl-D-glucoside fermentation test                        31. AMY    AMYgdaline fermentation                                                        amygdaline fermentation test                                        32. ARB    ARButine fermentation                                                          arbutine fermentation test                                          33. ESC    ESCuline                                                                       esculine hydrolysis test                                            34. SAL    SAlicine fermentation                                                          solicine fermentation test                                          35. TCEL   CELlubiose fermentation                                                        cellubiose fermentation test                                        36. MAL    MALtose fermentation                                                           maltose fermentation test                                           37. LAC    LACtose fermentation                                                           lactose fermentation test                                           38. MEL    MELibiose fermentation                                                         melibiose fermentation test                                         39. SAC    SACvharose fermentation                                                        sacvharose fermentation test                                        40. TRE    TREhalone fermentation                                                         trehalone fermentation test                                         41. INU    INUilne fermentation                                                           inuline fermentation test                                           42. MLZ    MeLeZitose fermentation                                                        melezitose fermentation test                                        43. RAF    RAFfinose fermentation                                                         raffinose fermentation test                                         44. GLG    GLycoGen fermentation                                                          glycogen fermentation test                                          45. XLT    XyLiTol fermentation                                                           xylitol fermentation test                                           46. DLY    D-LYxose fermentation                                                          D-lyxose fermentation test                                          47. DFU    D-FUcose fermentation                                                          D-fucose fermentation test                                          48. LFU    L-FUcose fermentation                                                          L-fucose fermentation test                                          49. DAR    D-ARabiol fermentation                                                         D-arabitol fermentation test                                        50. LAR    L-ARabiol fermentation                                                         L-arabitol fermentation test                                        51. GNT    GlucoNaTe                                                                      guconate test                                                       52. 2KG    2-keto-Gluconate                                                               2-keto-gluconate test                                               53. 5KG    5-keto-Gluconate                                                               5-keto-gluconate test                                               54. LDC    Lysine DeCarboxylase                                                           lysine decarboxylase test                                           55. ODC    Ornithine DeCarboxylase                                                        ornithine decarboxylase test                                        56. HYS    Hydrogen sulfide production                                                    hydrogen sulfide production test                                    57. TDA    Tyrptophane DesAminase                                                         tyrptophane desaminase test                                         58. IND    INDole production                                                              indole production test                                              59. V--P   acetion production                                                             acetion production test                                             60. GEL    GELatinase                                                                     gelatinase test                                                     61. NIT    reduction of NITrates                                                          reduction of nitrates test                                          ______________________________________                                    

12. Embodiment of lactobacilli-coding identification method:

Coding test items of lactobacilli ##STR12## Coding test items:

    ______________________________________                                         1. ARB     ARButine fermentation                                                          arbutine fermentation test                                          2. RIB     RIBose fermentation                                                            ribose fermentation test                                            3. NAG     N-Acetyl-Glucosamine fermentation                                              N-acetyl-glucosamine fermentation test                              4. DXY     D-XYlose fermentation                                                          D-xylose fermentation test                                          5. TRE     TREhalose fermentation                                                         trehalose fermentation test                                         6. SOR     SORbitol fermentation                                                          sorbitol fermentation test                                          7. SUC     SUCrose fermentation                                                           sucrose fermentation test                                           8. LAC     LACtose fermentation                                                           lactose fermentation test                                           9. CEL     CELlobiose fermentation                                                        cellobiose fermentation test                                        10. MBL    MELibiese fermentation                                                         melibose fermentation test                                          11. LAR    L-ARAbinose fermentation                                                       L-arabinose fermentation test                                       12. RHA    RHAmnose fermentation                                                          rhamnose fermentation test                                          13. DTA    D-TAgatose fermentation                                                        D-tagatose fermentation test                                        14. GNT    GlucoNaTe fermentation                                                         gluconate fermentation test                                         15. AMD    starch fermentation                                                            starch fermentation test                                            ______________________________________                                    

Complementary test item:

    ______________________________________                                         16. GLY    GLYcerol fermentation                                                          glycerol fermentation test                                          17. ERY    ERYthritol fermentation                                                        erythritol fermentation test                                        18. ARA    D-ARAbinose fermentation                                                       D-arabinose fermentation test                                       19. XYL    L-XYLose fermentation                                                          L-xylose fermentation test                                          20. ADO    ADOnitol fermentation                                                          adonitol fermentation test                                          21. MDX    β-Methyl-D-Xyloside fermentation                                          β-methyl-D-xyloside fermentation test                          22. GAL    GALactose fermentation                                                         galactose fermentation test                                         23. GLU    GLUcose fermentation                                                           glucose fermentation test                                           24. FRU    FRUctose fermentation                                                          fructose fermentation test                                          25. MNE    MaNnosE fermentation                                                           mannose fermentation test                                           26. SOS    SorboSe fermentation                                                           sorbose fermentation test                                           27. DUL    DULcitol fermentation                                                          dulcitol fermentation test                                          28. INO    INOnitol fermentation                                                          inonitol fermentation test                                          29. MAN    MANnitol fermentation                                                          mannitol fermentation test                                          30. MDM    α-Methyl-D-Mannoside fermentation                                        α-methyl-D-mannoside fermentation test                        31. MDG    α-Methyl-D-GLUcoside fermentation                                        α-methyl-D-glucoside fermentation test                        32. AMY    AMYgdaline fermentation                                                        amygdaline fermentation test                                        33. ESC    ESCuline                                                                       esculine hydrolysis test                                            34. SAL    SALicin fermentation                                                           salicin fermentation test                                           35. MAL    MALtose fermentation                                                           maltose fermentation test                                           36. INU    INUline fermentation                                                           inuline fermentation test                                           37. MLZ    MeLeZitose fermentation                                                        melezitose fermentation test                                        38. RAF    RAFfinose fermentation                                                         raffinose fermentation test                                         39. GLG    GLycoGene fermentation                                                         glycogene fermentation test                                         40. XLT    XyLiTol fermentation                                                           xylitol fermentation test                                           41. GEN    GENtiobiose fermentation                                                       gentiobiose fermentation test                                       42. TUR    D-TURanose fermentation                                                        D-turanose fermentation test                                        43. LYX    D-LYXose fermentation                                                          D-lyxose fermentation test                                          44. DFU    D-FUcose fermentation                                                          D-fucose fermentation test                                          45. LFU    L-FUcose fermentation                                                          L-fucose fermentation test                                          46. DAR    DARabitol fermentation                                                         D-arabitol fermentation test                                        47. LAR    L-ARabitol fermentation                                                        L-arabitol fermentation test                                        48. 2KG    2-keto-GLuconate                                                               2-keto-gluconate test                                               49. 5KG    5-keto-GLuconate                                                               5-keto-gluconate test                                               ______________________________________                                     

We claim:
 1. A bacteria identification method which uses coding test items and biochemical property, identifying papers that detect biological properties of bacterial strains characterized in that the coding test items in the bacteria-coding identification system are one of the following 12 kinds of combination;(1) the coding test items of identifying parenteral gram-negative bacilli are 15 tests of 2-keto-gluconate (2 KG), 3-hydroxy-benzoate (3 HB), 3-hydroxy-butyrate (OBU), citrate (CIT), L-proline (LPR), histosc (HIS), N-acetyl-D-glucosamine (NAG), glycogen (GLY), maltose (MAL), sucrose (SUC), D-melibiose (MEL), L-fucose (FUC), D-glucose (GLU), inositol (INO), and manitol (MAN); (2) the coding test items of identifying enteric gram-negative bacilli are 15 tests of oxidase (OXI), growth in MCK agar (MCK), motility (MOT), lysine decarboxylase (LDC), ornithint decarboxylase (ODC), arginine dihydrolase (ADH), indole production (IND), urease (URE), citrate utilization (CIT), glucose oxidation (GLO), mannitol fermentation (MAN), sucrose fermentation (SUC), rhamnose fermentation (RHA), amygdalin fermentation (AMY), and sorbitol fermentation (SOR); (3) the coding test items of identifying Enterobacteriaceae bacteria are 12 tests of lysine decarboxylase (LDC), ornitine decarboxylase (ODC), malonate utilization (MAU), urease (URE), cellobiose fermentation (CEL), D-sorbitol fermentation (SOR), L-arabinose fermentation (LAE), adoonitol fermentation (ADO), surcose fermentation (SUC), dulcitol fermentation (DUL), α-methyl-D-glucose fermentation (AMG) and D-mannitol fermentation (MAN); (4) the coding test items of identifying anerobic bacteria are 14 tests of gram stain (GRS), sporulation (SPO), α-arabirosidase (AAR), β-glucosidase (BGL), β-galactosidase (BGA), β-glucuronidase (BGU), laucine arylamidaes (LAA), proline arylamidase (PRA), alakaline phosphatase (ALP), indole production (IND), mannose fermentation (MAS), and β-N-acetyl-glycosaminidase (NAG); (5) the coding test items of identifying neisserial hemophilus are 9 tests of catalose (CAT), growth in Martin-Thayer medium (MTM), lucose fermentation (GLU), maltose fermentation (MLT), β-glucosidase (BGL), phenylphosphonate (OPS), glycine anglamidase (GLY), γ-glutamyl arylamidase (GGT), proline arylamidase (PRO) and reduction of resazurin (RES); (6) the coding test items of identifying compylbacter are 9 tests of catalase (CAT), reduction of nitrate (NIT), urease (URE), succinate assimilation (SUT), acetate assimilation (ACE), hippurate hydrolysis (HIP), hydrogen sulfide production (HYS), γ-glycotamyl transferase (GGT) and alkaline phosphatose (ALP); (7) the coding test items of identifying yeast-like fungi are 15 tests of 2-keto-gluconate (2 KG), actidine (ACT), erythritol (ERY), mannitol (MAN), inositol (INO), N-acetyl-glucosamine (NAG), L-arabinose (LAR), galatose (GAL), raffinose (RAF), cellobose (CEL), lactose (LAC), maltose (MAL), melibiose (MEL), trehalsoe (TRE) and esculin (ESC); (8) the coding test items of identifying corynebacteria are 9 tests of reduction of nitrate (NIT), urease (URE), esculin hydrolysis (ESC), L-maltose fermentation (MAL), sucrose fermentation (SUC), ribose fermentation (RIB), lactose fermentation (LAC), α-glucosidase (AGL) and β-galactosidase (BGA); (9) the coding test items of identifying micrococcaceae bacteria are 12 tests of urease (URE), reduction of nitrte (NIT), 3-hydroxyl-butanone production (HYB), arginine dihydrolase (ADH), ornithine decarboxylase (ODC), alkaline phosphatase (ALP), β-galactosidase (BGA), β-glucuronidase (BGU), glucose fermentation (GLF), sucrose fermentation (SUC), mannitol fermentation (MAN) and trehalose fermentation; (10) the coding test items of identifying Streptoccaceae bacteria are 13 tests of hemdysis (HEM), 3-hydroxy-butanone production (HYB), hippurate hydrolysis (HIP), arginine dihydrolase (ADH), L-leucine arylamidase (LAA), β-glucuronidase (BGU), β-galactosidase (BGA), ribose fermentation (RIB), raffinose fermentation (RAF), sorbitol fermentation (SOR), L-arabinose fermentation (LAR) and trehalose fermentation (TRE); (11) the coding test items of identifying bacilli are: 15 tests of arginine dihydrolase (ADH), urease (URE), citrate utilization (CIT), β-glucosidase (BGL), starch dyrolysis (AMD), L-arabinose fermentation (LAR), ribose fermentation (RIB), sorbitol fermentation (SOR), D-tagatose fermentation (DTA), N-acetyl-glucosamine fermentation (NAG), fructose fermentation (FRU), mannose fermentation (MAS), galactose fermentation (GAL), gentiobiose fermentation (GEN) and α-methyl-D-mannoside fermentation (MDM); (12) the coding test items of identifying lactobacilli are 15 tests of arbutine fermentation (ARB), ribose fermentation (RIB), N-acetyl-glucosamine fermentation (NAG), D-xylose fermentation (DXY), trehalose fermentation (TRE), sorbitol fermentation (SOR), sucrose fermentation (SUC), lactose fermentation, (LAC), celloblose fermentation (CEL), melibiose fermentation (MEL), L-arabinose fermentation (LAR), rhamnose fermentation (RHA), D-tagatose fermentation (DTA), gluconate fermentation (GNT) and strach hydrolysis (AMD) and that thekind of bacteria biochemical properties-identifying papers that detect biological properties of bacteria according to the bacteria identification method are characterized in that the bacteria biochemical test mediums added in the paper-holes are one of the following 12 groups;(1) the biochemical properties-identifying papers of parenteral gram-negative bacilli are assimilation test mediums of 2-keto-gluconate (2 KG), 3-hydroxy-benzoate (3 HB), 3-hydroxy-butyrate (OBU), citrate (CIT), L-proline (LPR), histose (HIS), N-acetyl-D-glucosamine (NAG), glycogen (GLY), maltose (MAL), sucrose (SUC), D-melibiose (MEL), L-fucose (FUC), D-glucose (GLU), inositol (INO) and manitol (MAN); (2) the biochemical properties-identifying papers of enteric gram-negative bacilli are test mediums of lysine decarboxylas (LDC), ornithint decarboxylase (ODC), arginine dihydrolase (ADH), indole production (IND), urease (URE), citrate utilization (CIT), glucose oxidation (GLO), mannitol fermentation (MAN), sucrose fermentation (SUC), rhammose fermentation (RHA), amygdelin fermentation (AMY) and sorbitol fermentation (SOR); (3) the biochemical properties-identifying papers of Enterobacteriaceae bacteria are test mediums of lysine decarboxylase (LDC), ornitine decarboxylase (ODC), malonate utilization (MAU), urease (URE), cellobiose fermentation (CEL), D-sorbitol fermentation (SOR), L-arabinose fermentation (LAR), adonitol fermentation (ADO), sucrose fermentation (SUC), dulcitol fermentation (DUL), α-methyl-D-glucoside fermentation (ANG) and D-mannitol fermentation (MAN); (4) the biochemical properties-identifying papers of anaenobic bacteria are test mediums of α-glucosidase (AGL,), α-galactosidase (AGA), α-arabinosidase (AAR), β-glucosidase (BGL), β-galactosidase (BGA), β-glucuronidase (BGU), leucine anglamidase (LAA), proline arylamidase (PRA), alkaline phosphatase (ALP), indole production (IND), mannose fermentation (MAS) and N-acetyl-D-glucosaminidase (NAG); (5) the biochemical properties-identifying papers of neisserial hemophilus are test mediums of growth in Martin-Thayer medium (MTM), glucose fermentation (GLU), maltose fermentation (MLT), β-glucosidase (BGL), phenylphosphonate (OPS), glycine arylamidase (GLY) Υ-glutamyl arylamidase (GGT), proline arylamidase (PRO), and reduction of resazurin (RES); (6) the biochemical properties-identifying papers of camylobacter are test mediums of catalase (CAT), reduction of intrate (NIT), urease (URE), succinate assimilation (SUT), acetate assimulation (ACE) hippurate hydrolysis (HIP), hydrogen sulfide production (HYS), Υ-glutamyl transferase (GGT) and alkaline phosphatase (ALP); (7) the biochemical properties-identifying papers of yeast-like fungi are assimilation test mediums of 2-keto-gluconate (2 KG), actidione (Act), erythritol (ERY), mannitol (MAN), inositol (INO), N-acetyl-glucosamine (NAG), L-arabinose (LAR), galactose (GAL), raffinose (RAF), cellobose (CEL), lactose (LAC), maltose (MAL), malibiose (MEL), trehalose (TRE) and esculin (ESC); (8) the biochemical properties-identifying papers of corynebacteria are the mediums of redact of intrate (MI), urease (URE), esculin hydrolysis (ESC), L-maltose fermentation (MAL), sucrose fermentation (SUC), riborse fermentation (RIB), lactose fermentation (LAC), α-glucosidase (AGL), and β-galactosidase (BGA); (9) the biochemical properties-identifying papers of micrococcaceae aceria are test medium of urease (URE), reduction of nitrate (NIT), 3-hydroxyl-butanone production (HYB), arginine dihyrolase (ADH), ornithinic decarboxylase (ODC), alkaline phosphatase (ALP), β-galactosidase (BGA), β-glucuronidase (BGU), glucose fermentation (GLF), sucrose fermentation (SUC), mannitol fermentation (MAN) and trehalose fermentation (TRE); (10) the biochemical properties-identifying papers of Streptoccaceae bacteria are test mediums of 3-hydroxy-butanone production (HYB), hippurate hydrolysis (HIP), arginine dihydrolase (ADH), α-leucine arylamidase (LAA), b-glucuronidase (BGU), β-galactosidase (BGA), ribose fermentation (RIB), raffinose fermentation (RAF), sorbitol fermentation (SOR), L-arabinose fermentation (LAR) and trehalose fermentation (TRE); (11) the biochemical properties-identifying papers of bacilli are test mediums of arginine dihydrolase (ADH), urease (URE), citrate utilization (CIT), β-glucosidase, (BGL), starch hydrolysis (AMD), L-arabinose fermentation (LAR), ribose fermentation (RIB), sorbitol fermentation (SOR), D-tagatose fermentation (DTA), N-acetyl-glucosamine fermentation (NAG), fructose fermentation (FRU), mannose fermentation (MAS), galactose fermentation (GAL), gentiobiose fermentation (GEN) and α-methyl-D-mannoside fermentation (MDM); and (12) the biochemical properties-identifying papers of lactobacilli are test mediums of arbutine fermentation (ARB), ribose fermentation (RIB), N-acetyl-glucosaine fermentation (NAG), d-xylose fermentation (DXY), trehalose fermentation (TRE), sorbitol fermentation (SOR), sucrose fermentation (SUC), lactose fermentation (LAC), cellobiose fermentation (CEL), melibiose fermentation (MEL), L-arabinose fermentation (LAR), rhamnose fermentation (RHA), D-tagotose fermentation (DTA), gluconate fermentation (GNT) and starch hydrolysis (AMD), said method comprising the steps of:(a.) selecting the coding test items for bacteria identification according to known bacteria biological properties; (b.) preparting bacteria biochemical property, identifying papers based upon the selected coding test items; (c.) using the bacteria biochemical, identifying papers to detect the biochemical properties of the bacterial strain to be identified.
 2. A bacteria identification apparatus using the bacteria identification method defined in claim 1 comprising:image sampling unit, input unit, processing unit, storage unit, output unit and software system, characterized in that the storage unit use the non-volatile semi-conductor memory to store software and data.
 3. An bacteria identification apparatus according to claim 2, characterized in that said non-volatile semiconductor memory is any one of programmable read-only memory, erasable-programmable read-only memory or flash memory.
 4. An bacteria identification apparatus according to claim 2, characterized in that said output unit is color CRT or liquid crystal display, which can display biological properties of bacteria in the form of sketch image with the support of software.
 5. An bacteria identification apparatus according to claim 2, characterized in that said image sampling unit has CCD camera and image sampling means; with the support of software, said CCD camera transfer color-changing image which embodies bacteria biological properties into video signal being transfered into image sampling means; said image sampling means transfers the video signal from CCD camera into digital signal to be processed by processing unit.
 6. An bacteria identification apparatus according to claim 2, characterized in that said image sampling unit, input unit, processing unit, storage unit and output unit assembled together as a whole. 